通过对马尾橙毛虫质型多角体病毒的增殖、纯化，获得一株单一类型的质型多角体病毒。提纯的病毒粒子经 SDS一酚抽提，琼脂糖凝胶电泳分离基因组dsPdgDA，回收纯化第十片段SIO。SIO经DMSO变性，逆转录合成cDNA 第一链，PCR扩增后，克隆在DGE T载体上。对重组子进行限制性内切酶分析及序列测定，结果表明，克隆片段全 长763b口，起始密码AUG位于3—5残基，终止密码UGA位于747—749残基。推测Dt,C2V多角体蛋白基因编码248 个氨基酸的多肽，分子量28kD。和家蚕质型多角体病毒(BmCPV)多角体蛋白基因相比较，核苷酸和编码氨基酸序 列同源性分别为89，3％和97．6％。CPVs belong to the genus Cypovirus in the family Reoviridae and infect midgut epithelial cells of the wide range of insects.For the synthesis of cDNA of Dendrolimus punctatus CPV (DpCPV) polyhedrin gene,the primers were constructed on the basis of the terminal RNA sequence of BmCPV segment 10,After RT PCR,the amplified cDNA was cloned into the pGEM T.The S10 cDNA of DpCPV was found to be 763 nucleotides in length with a single open reading frame in one strand capable of coding a predicted protein of 248 residues (Mr of 28411).Comparison of the nucleotide sequence of the polyhedrin gene of DpCPV with that of BmCPV showed that nucleotides homology is 89.3%,but there are only 6 amino acid differences between the two CPVs.

CPVs belong to the genus Cypovirus in the family Reoviridae and infect midgut epithelial cells of the wide range of insects.For the synthesis of cDNA of Dendrolimus punctatus CPV (DpCPV) polyhedrin gene,the primers were constructed on the basis of the terminal RNA sequence of BmCPV segment 10,After RT PCR,the amplified cDNA was cloned into the pGEM T.The S10 cDNA of DpCPV was found to be 763 nucleotides in length with a single open reading frame in one strand capable of coding a predicted protein of 248 residues (Mr of 28411).Comparison of the nucleotide sequence of the polyhedrin gene of DpCPV with that of BmCPV showed that nucleotides homology is 89.3%,but there are only 6 amino acid differences between the two CPVs.