从患流行病的鳜鱼脾脏组织超微切片中观察到大量的病毒颗粒。该完整病毒颗粒直径约 135nm± 10 ,具包膜。成熟病毒核壳体约 90nm± 5 ,包膜厚度约 18nm± 3,核壳体与包膜间的非电子致密区约有 2 7nm± 2。通过对不同发病阶段发病鳜鱼脾脏组织切片的电镜观察 ,在感染初期的鳜鱼脾脏组织观察到病毒的吸附及典型的内吞入侵方式 ,在感染中后期的脾脏组织细胞质内观察到病毒发生基质及病毒核壳、包膜形成与病毒的释放。此外 ,在染病鳜鱼的肾、心、肝、及鳃组织亦观察到相同结构的病毒粒子。回接实验证实该病毒为引起鳜鱼暴发流行病的病原 The structure and morphogensis of Mandarin fish( Siniperca chuats ) virus were studied.The matured virons were about 135nm±10 in diameter with envelope outside.The intact virus is composed of three parts,its nucleocapsid was about 90nm±5,and the envelope was around 18nm±3,the air space between core and envelope was about 27nm±2?The morphogenesis of the virus was observed in the ultra thin section of spleen tissue cells with outbreak lethal disease,which were collected in different stages of infected mandarin fish.At early stage infected fish section,it showed typical virus entry into cell cytoplasm via viropexis.There were many viromatrix,unmatured,matured and viron released phases,which were found in middle and late phase of diseased fish.In addtion,the same virions were also detected in diseased kidney,liver,heart and gill tissue.Artificial infected fish also caused acute lethal disease with same virus found in its tissue section.

The structure and morphogensis of Mandarin fish( Siniperca chuats ) virus were studied.The matured virons were about 135nm±10 in diameter with envelope outside.The intact virus is composed of three parts,its nucleocapsid was about 90nm±5,and the envelope was around 18nm±3,the air space between core and envelope was about 27nm±2?The morphogenesis of the virus was observed in the ultra thin section of spleen tissue cells with outbreak lethal disease,which were collected in different stages of infected mandarin fish.At early stage infected fish section,it showed typical virus entry into cell cytoplasm via viropexis.There were many viromatrix,unmatured,matured and viron released phases,which were found in middle and late phase of diseased fish.In addtion,the same virions were also detected in diseased kidney,liver,heart and gill tissue.Artificial infected fish also caused acute lethal disease with same virus found in its tissue section.