M odel Establishment of the Cell Persistently Infected by a Field—isolated Virulent Strain of CSFV

YING Song-cheng; ZHENG Cong; QU San; ZHANG Chu; WANG Zai

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February 2002

YING Song-cheng, ZHENG Cong, QU San, ZHANG Chu and WANG Zai. M odel Establishment of the Cell Persistently Infected by a Field—isolated Virulent Strain of CSFV[J]. Virologica Sinica, 2002, 17(1): 77-81.

Citation: YING Song-cheng, ZHENG Cong, QU San, ZHANG Chu, WANG Zai. M odel Establishment of the Cell Persistently Infected by a Field—isolated Virulent Strain of CSFV .VIROLOGICA SINICA, 2002, 17(1) : 77-81.

猪瘟病毒野毒株持续感染细胞模型的建立

1.
1．武汉大学生命科学学院，湖北武汉430072
2.
中医兽药监察所．北京100081

摘要

摘要：采用猪瘟病毒野毒珠(CSFV39)感染猪肾传代细胞系PK一15，经连续传79代，建立了稳定的病毒持续感染细胞模型．获得CSFV39一PK15细胞株。用免疫荧光、RT-PCR检测、透射电镜跟踪观察了CSFV39一PK15细胞株连续传代中病毒在细胞内的存在情况。结果表明第9，29，79代细胞仍有猪瘟病毒存在，表现出病毒持续感染的基本特征。这为课八研究猪瘟病毒持续感染机理奠定了基础。
- 猪瘟病毒株CSFV39
- , 持续感染
- , 细胞模型
- , RT-PCR

M odel Establishment of the Cell Persistently Infected by a Field—isolated Virulent Strain of CSFV

1.
1．College of Life Sciences，Wuhan University，Wuhan 430072．China 2．Ch ina Institute of Veterinary Drug Control，Beljing 100081，Ch ina

Abstract

Abstract：A porcine kidney cell line(PK一15)was infected by a field—isolated virulent strain of classical swine fever virus(CSFV39) After the infected cells had subeultured 79 passages for near eight months．a steady modeI of the eell persistently infected by virus has been establisheel，hence the CS— FV39．PK15 cell line was procured．In this experiment immunofluorescenee，RT-PCR assay and elec— tron microscopy observation were used to identify the basic viral fearums in the CSFV39一PK15 cel1． The results showed the 9th，29th．79th CSFV39一PK15 cell pasages still harhored CSFV and they ha d the radical characteristics of viral persistent infectlon．This endeavor should be the fotmdation for the further research of CSFV persistent infection．
- Clasical swine fever virus(CSFV39)
- , Persistent infection
- , CelI model
- , RT-PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

M odel Establishment of the Cell Persistently Infected by a Field—isolated Virulent Strain of CSFV

1. 1．College of Life Sciences，Wuhan University，Wuhan 430072．China 2．Ch ina Institute of Veterinary Drug Control，Beljing 100081，Ch ina

Abstract: Abstract：A porcine kidney cell line(PK一15)was infected by a field—isolated virulent strain of classical swine fever virus(CSFV39) After the infected cells had subeultured 79 passages for near eight months．a steady modeI of the eell persistently infected by virus has been establisheel，hence the CS— FV39．PK15 cell line was procured．In this experiment immunofluorescenee，RT-PCR assay and elec— tron microscopy observation were used to identify the basic viral fearums in the CSFV39一PK15 cel1． The results showed the 9th，29th．79th CSFV39一PK15 cell pasages still harhored CSFV and they ha d the radical characteristics of viral persistent infectlon．This endeavor should be the fotmdation for the further research of CSFV persistent infection．