Nucleotide Sequence of Non-structural Protein P3 Coding Region of Foot-and-mouth Disease Virus

IAU Zai—xin; ZHAO Q．_ZU; ZHANG Xian—sheng; JIANG Peng—fei; CHANG Hui—yong; XIE Qing-ge

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April 2002

IAU Zai—xin, ZHAO Q．_ZU, ZHANG Xian—sheng, JIANG Peng—fei, CHANG Hui—yong, XIE Qing-ge, Nucleotide Sequence of Non-structural Protein P3 Coding Region of Foot-and-mouth Disease Virus[J]. Virologica Sinica, 2002, 17(2): 153-157.

Citation: IAU Zai—xin, ZHAO Q．_ZU, ZHANG Xian—sheng, JIANG Peng—fei, CHANG Hui—yong, XIE Qing-ge, . Nucleotide Sequence of Non-structural Protein P3 Coding Region of Foot-and-mouth Disease Virus .VIROLOGICA SINICA, 2002, 17(2) : 153-157.

口蹄疫病毒非结构蛋白P3区的基因序列及分析

1.
中国农业科学院兰州兽医研究所，兰州730046

摘要

摘要：以日蹄疫Akesu／58分离株的53代牛舌皮病料为材料，采用RT PCR法，扩增和克隆了两个约1．5kb的DNA 片段。桉酸序列测得结果对接后，涵盖了全部P3区的基因序列。日蹄疫Akesu／58分离株基因组P3区的核酸序列共计2，724nt，包括一个终止密码子TAA，共编码907个氨基酸；其中非结构蛋白3A的基因是459nt，编码153个氨基酸；3个3B(VPg)基因分别是69 72和72nt，氨基酸分别为23、24和24；3c是639nt，213个氨基酸；3D是 1，413nt，471个氨基酸各蛋白间由Glu／Gly(Serl连接。序列比较显示：3A的C端易变，其它区的变易呈零星散在。
- 口蹄疫病毒
- , 非结构蛋白：P3区基因

Nucleotide Sequence of Non-structural Protein P3 Coding Region of Foot-and-mouth Disease Virus

1.
Lanzhou Veterinary Research Institute of CAAS，Lanzhou 730046，China

Abstract

Abstract：Two i．5kb DNA were amplified by reverse transcription and PCR(RT—PCR)from the ep— ithelial tongue tissue of cattle infected with FMDV akesu／58 strain after 53 passages，and then cloned A continuous 2，724 nuclco tlde sequence spanning the P3 codiiag region was obtained，including a end— ing codon TAA 907 amino acids were encoded．3A protein gene was composted of 459 nuclcotides． encoding 153 amino acids Three 3B(VPg)genes had 69．72 and 72 nuclcotides and encoded 23，24 and 24 amino acids， respectively 639 nuclcotides and 213 amino acids were attributed to 3C and 1，4 13 nucleotides and 471 amino acids to 3D．All proteins were linked by cleavage sites G1u／Gly (Set)．In addition，the varlateCterminal part of 3A protein hasbeenidentified by sequence alignment with other FMDV strains．
- Foot—and—mouth disease virus
- , Non—structura[protein

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Nucleotide Sequence of Non-structural Protein P3 Coding Region of Foot-and-mouth Disease Virus

1. Lanzhou Veterinary Research Institute of CAAS，Lanzhou 730046，China

Abstract: Abstract：Two i．5kb DNA were amplified by reverse transcription and PCR(RT—PCR)from the ep— ithelial tongue tissue of cattle infected with FMDV akesu／58 strain after 53 passages，and then cloned A continuous 2，724 nuclco tlde sequence spanning the P3 codiiag region was obtained，including a end— ing codon TAA 907 amino acids were encoded．3A protein gene was composted of 459 nuclcotides． encoding 153 amino acids Three 3B(VPg)genes had 69．72 and 72 nuclcotides and encoded 23，24 and 24 amino acids， respectively 639 nuclcotides and 213 amino acids were attributed to 3C and 1，4 13 nucleotides and 471 amino acids to 3D．All proteins were linked by cleavage sites G1u／Gly (Set)．In addition，the varlateCterminal part of 3A protein hasbeenidentified by sequence alignment with other FMDV strains．