WAN Yan—ping, W U Yi—mou, TANG Li—zhi and LIAO Duan—fang. Interaction of Adenovirus E 1 B 55kD Oncoproteins with hDaxx[J]. Virologica Sinica, 2002, 17(3): 230-233.
Citation:
WAN Yan—ping, W U Yi—mou, TANG Li—zhi, LIAO Duan—fang.
Interaction of Adenovirus E 1 B 55kD Oncoproteins with hDaxx .VIROLOGICA SINICA, 2002, 17(3)
: 230-233.
腺病毒EIB 55kD癌蛋白与hDaxx的相互作用
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摘要
为了探讨腺病毒(adenovirus,Ad)E1B 55kD癌蛋白(Ad E1B 55kD)打破hDaxx和PML共定位细胞核的作用 机制,本文利用体内外共免疫沉淀反应研究Ad EIB 55kD与hDaxx的结合反应,并通过酵母双杂交体系测定两种 蛋白质的相互作用及其作用的氨基酸残基序列。结果显示:Ad2 EIB 55kD 通过C端58个氨基酸(aa)与hDaxx结 合并发生相互作用。Adl2 EIB 55kD与hDaxx结合需全序列aa及其构象。共免疫沉淀反应和Western blot结果证 实Ad2/5或Adl2 EIB 55kD能在体内外与hDaxx直接结合。
Interaction of Adenovirus E 1 B 55kD Oncoproteins with hDaxx
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Abstract
Abstract:To question the reason of CO—localization of hDaxx and PM L in PODs disrupted by Aden— ovirus(Ad)E1 B 55一kilodalton oncoproteins(Ad E1 B 55kD),the interaction of Ad E1 B 55kD with hDaxx was analyzed by the yeast two-hybrid assay, and the direct binding of Ad E1 B 55kD with hDaxx was studied by coimm unoprecipitation reaction in vivo or in vitro.The results show that Ad E1B 55kD oncoproteins interact with hDaxx by binding the C-terminus 58 am ino acids of A d2 E1B 55kD or the full length of Adl2 E1B 55kD.Ad2/5 or Adl2 E1B 55kD directly binds hDaxx in vivo or in vitro.
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Proportional views
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