Cucum ber M osaic Virus and Tobacco M osaic Virus Proliferation in Transgenic Chili Peppers and its Protoplast

XU Bing—liang; SHANG Hong—sheng; WANG Xu

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June 2002

XU Bing—liang, SHANG Hong—sheng and WANG Xu. Cucum ber M osaic Virus and Tobacco M osaic Virus Proliferation in Transgenic Chili Peppers and its Protoplast[J]. Virologica Sinica, 2002, 17(3): 243-247.

Citation: XU Bing—liang, SHANG Hong—sheng, WANG Xu. Cucum ber M osaic Virus and Tobacco M osaic Virus Proliferation in Transgenic Chili Peppers and its Protoplast .VIROLOGICA SINICA, 2002, 17(3) : 243-247.

黄瓜花叶病毒和烟草花叶病毒在双抗转基因线辣椒内的增殖

徐秉良 ,
商鸿生 ,

1.
1．甘肃农业大学植保系，甘肃兰州730070
2.
西北农业大学植保系，陕西杨凌712100

摘要

本试验以转化CMV—CP和TMv—CP基因的转基因线辣椒纯合系植株作为研究试材，比较了单独或混合接种 CMV和TMV后，转化线辣椒的抗病性表达特点，并测定了两种病毒在植株体内的病毒含量。结果表明：转化线辣椒不仅能抵抗CMV和TMv 的单独侵染，而且还能抵抗CMV 和TMv的复合侵染。转化线辣椒表现为系统症状延迟出现7—15d，显症株率和病害严重度级别大幅度降低，CMV和TMv在接种叶、新生叶中的病毒含量明显减低。转基因线辣椒原生质体作为研究试材接种CMV，测定病毒含量结果表明：CMV 病毒的增殖在转基因线辣椒原生质体内受到明显抑制。在CMV 接种浓度为40t~g／mL，感染原生质体48h后，CP(一)植株原生质体内CMV是CP (+)的4．2倍。这一结果揭示了转基因线辣椒具有抑制病毒增殖的抗病性。
- 转基因线辣椒
- , 抗病性
- , CMV
- , TMV
- , 原生质体
- , 病毒增殖

Cucum ber M osaic Virus and Tobacco M osaic Virus Proliferation in Transgenic Chili Peppers and its Protoplast

1.
1．Plant protection department of Gausu Agricul tural University，Lanzhou 730070，China
2.
Plant Protection Department of NorthzL—estern Agricultural University。Yanglin 712100，China

Abstract

Transgenic chili pepper plant expressing CM V and TM V coat protein gene were infected with CM V and TM V to com paring the characteristic of CP—M R in transgenic chili pepper and virus content in inoculated plants．The results showed that the transgenic chili pepper exhibited high level of coat protein—mediated resistance to both CM V and TM V ．The developm ent of systemic sym ptom was 7 ～ 15 days delayed，percentages of plants showing sym ptoms and severity of disease sym ptom in inocu— lated plants were strongly reduced，and virus accumulat and spread were obviously repressed in inocu— lated and lewly—developed leaves in CP(+)plants challenged with CMV and TMV．The protoplasts from transgenic chili pepper expressing CM V—CP and TMV—CP were infected with CMV virion．The results showed that protoplasts from transgenic chili pepper can also inhibit the virus proliferation．The protoplasts were infected with 40btg／mL virus concentration，48 hours later，the amount of virus in con— trol plant protoplasts was 4．2 times than that in CP(+)plant protoplasts．This results demonstrated that the resistance characteristic of transgenic chili pepper was tO reduce virus proliferation in plants．
- Transgenic chili peper
- , Resistance characteristic
- , CMV
- , TMV
- , Protoplast
- , V irus Prolifera-tion

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cucum ber M osaic Virus and Tobacco M osaic Virus Proliferation in Transgenic Chili Peppers and its Protoplast

1. 1．Plant protection department of Gausu Agricul tural University，Lanzhou 730070，China
2. Plant Protection Department of NorthzL—estern Agricultural University。Yanglin 712100，China

Abstract: Transgenic chili pepper plant expressing CM V and TM V coat protein gene were infected with CM V and TM V to com paring the characteristic of CP—M R in transgenic chili pepper and virus content in inoculated plants．The results showed that the transgenic chili pepper exhibited high level of coat protein—mediated resistance to both CM V and TM V ．The developm ent of systemic sym ptom was 7 ～ 15 days delayed，percentages of plants showing sym ptoms and severity of disease sym ptom in inocu— lated plants were strongly reduced，and virus accumulat and spread were obviously repressed in inocu— lated and lewly—developed leaves in CP(+)plants challenged with CMV and TMV．The protoplasts from transgenic chili pepper expressing CM V—CP and TMV—CP were infected with CMV virion．The results showed that protoplasts from transgenic chili pepper can also inhibit the virus proliferation．The protoplasts were infected with 40btg／mL virus concentration，48 hours later，the amount of virus in con— trol plant protoplasts was 4．2 times than that in CP(+)plant protoplasts．This results demonstrated that the resistance characteristic of transgenic chili pepper was tO reduce virus proliferation in plants．