A Research of The Stability Based on A Cell M odel Persistently Infected By A Field．isolated Virulent Strain of CSFV

XU zhen; YING Song; SHEN Chao; QU San; ZHENG Cong; WANG Zai

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June 2003

XU zhen, YING Song, SHEN Chao, QU San, ZHENG Cong and WANG Zai. A Research of The Stability Based on A Cell M odel Persistently Infected By A Field．isolated Virulent Strain of CSFV[J]. Virologica Sinica, 2003, 18(3): 271-274.

Citation: XU zhen, YING Song, SHEN Chao, QU San, ZHENG Cong, WANG Zai. A Research of The Stability Based on A Cell M odel Persistently Infected By A Field．isolated Virulent Strain of CSFV .VIROLOGICA SINICA, 2003, 18(3) : 271-274.

猪瘟病毒野毒株持续感染细胞模型的稳定性研究

1.
1．武汉大学生命科学学院，湖北武汉430072：2．中国兽药监察所，北京100081

摘要

以本实验室建立的CSFV39-PK15持续感染细胞模型为实验材料,综合运用免疫荧光、RT-PCR、流式细胞仪,对其稳定性进行研究。实验结果均表明,该细胞模型有着良好的稳定性。即使在连续传至128代的CSFV39-PK15传代细胞中,CSFV仍持续存在:呈免疫荧光抗体反应阳性和RT-PCR检测阳性。同时,该细胞与正常的PK-15细胞相比,细胞周期无显著差异。通过同段序列的同源性比较,发现CSFV39与CSFV石门株的同源性最高,达99.02%。
- CSFV39-PK15细胞模型
- , 持续感染
- , 细胞周期
- , 稳定性

A Research of The Stability Based on A Cell M odel Persistently Infected By A Field．isolated Virulent Strain of CSFV

1.
{1．College oflife sciences．Wuhan University,Wuhan 430072，China

Abstract

We jointly applied immunofluorescence technique, RT-PCR assay and flow cytometer to evaluate the stability of a cell model persistently infected by Classical swine fever virus (CSFV39), which was established by our laboratory and named CSFV39-PK15. The experiment results indicated that this cell model was quite stable. Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results. We also found there was no significant difference in their cell cycle between normal control PK-15 and CSFV39-PK15. Sequence homology analysis of the same fragment revealed that CSFV39 had the highest homology compared with Shimen strain, amounting to 99.02%.
- CSFV39-PK15 cell model Persistent infection Cell cycle Stability

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A Research of The Stability Based on A Cell M odel Persistently Infected By A Field．isolated Virulent Strain of CSFV

1. {1．College oflife sciences．Wuhan University,Wuhan 430072，China

Abstract: We jointly applied immunofluorescence technique, RT-PCR assay and flow cytometer to evaluate the stability of a cell model persistently infected by Classical swine fever virus (CSFV39), which was established by our laboratory and named CSFV39-PK15. The experiment results indicated that this cell model was quite stable. Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results. We also found there was no significant difference in their cell cycle between normal control PK-15 and CSFV39-PK15. Sequence homology analysis of the same fragment revealed that CSFV39 had the highest homology compared with Shimen strain, amounting to 99.02%.