提要：用电镜负染方法在发病的贻贝内脏团、鳃、外套膜组织匀浆中观察到一种球形病毒粒子，其直径为80～ 200nm，具囊膜。上述组织超薄切片发现在其结缔组织细胞质中均存在该病毒粒子，带双层囊膜，有“封入体” 结构，未见包涵体。在未发病的杂色蛤、香螺、牡蛎等内脏团中也观察到少量同种病毒粒子。用带病毒贻贝组织 匀浆投喂和划伤感染不带病毒的绣凹螺，使之成为带病毒状态。采用蔗糖梯度离心法，25 000 r／min，离心3h， 可于20％~30％蔗糖层间获得病毒沉淀带。 关键词：贻贝；球形病毒；病毒纯化； 电镜检提要：用电镜负染方法在发病的贻贝内脏团、鳃、外套膜组织匀浆中观察到一种球形病毒粒子，其直径为80～ 200nm，具囊膜。上述组织超薄切片发现在其结缔组织细胞质中均存在该病毒粒子，带双层囊膜，有“封入体” 结构，未见包涵体。在未发病的杂色蛤、香螺、牡蛎等内脏团中也观察到少量同种病毒粒子。用带病毒贻贝组织 匀浆投喂和划伤感染不带病毒的绣凹螺，使之成为带病毒状态。采用蔗糖梯度离心法，25 000 r／min，离心3h， 可于20％~30％蔗糖层间获得病毒沉淀带。

Abstract：A lot of spherical virions were observed in internal organiT．ation such as gill and mantellum of the diseased Mytilus edulis with negative staining method．The diameter of V on is around 8()_一 200nm with dual unit membran e．Sam e virions were observed in connective tissuese of infected organ s with ultrathin section electronmicroscopic observ ation．Th e“occlusion body’’were observed in cell matrix an d no inclusion bod y was present．A few same virions were observed in internal organization of normal Ruditapes variega~，Neptunea cumingi an d Dstrea lalienwhanensis with negative staining method．，nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses from diseased Mytilus edulis through mouth and injured section on skin．Th e virus was purified from the diseased tissue by sucrose gradient centrifugation with 25 0oo r／min．3h．Th e virus sediment lies between 2O％ ～ 3O％ of sucrose． Key word：Mytilus edulis；SphericalAbstract：A lot of spherical virions were observed in internal organiT．ation such as gill and mantellum of the diseased Mytilus edulis with negative staining method．The diameter of V on is around 8()_一 200nm with dual unit membran e．Sam e virions were observed in connective tissuese of infected organ s with ultrathin section electronmicroscopic observ ation．Th e“occlusion body’’were observed in cell matrix an d no inclusion bod y was present．A few same virions were observed in internal organization of normal Ruditapes variega~，Neptunea cumingi an d Dstrea lalienwhanensis with negative staining method．，nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses from diseased Mytilus edulis through mouth and injured section on skin．Th e virus was purified from the diseased tissue by sucrose gradient centrifugation with 25 0oo r／min．3h．Th e virus sediment lies between 2O％ ～ 3O％ of sucrose．