YAN Ran, SHEN Chao, LEI Lei, ZHENG Cong—yi, XIAO Geng—fu, GUO De—yin, ZHU Ymg and YAN Hui一min. SARS-CoV Infection Induces Apoptosis of Vero E6[J]. Virologica Sinica, 2003, 18(6): 541-543.
Citation:
YAN Ran, SHEN Chao, LEI Lei, ZHENG Cong—yi, XIAO Geng—fu, GUO De—yin, ZHU Ymg, YAN Hui一min.
SARS-CoV Infection Induces Apoptosis of Vero E6 .VIROLOGICA SINICA, 2003, 18(6)
: 541-543.
SARS—CoV感染Vero E6细胞诱导细胞凋亡
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1.
武汉大学生命科学学院，湖北武汉，430072
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摘要
摘要：为了确定SARS冠状病毒(SARS—CoV)感染Vero E6是否引起细胞凋亡，我们利用细胞DNA琼脂糖电泳， 感染细胞的间接荧光染色和Hoechst 33258细胞核染色，以及流式细胞仪分析等方法证明了SARS．CoV感染的Vexo E6具有典型的凋亡细胞学和生物化学特征。实验证明具有细胞凋亡特征的所有细胞均为处于感染晚期的细胞。表 现明显细胞病变(CPE)的细胞大多已经出现核质凝缩或形成凋亡小体进入细胞凋亡的过程。可以断定SARS．CoV 感染VeroE6细胞诱发了细胞凋亡。
Abstract：To determine if SARS—associated coronavirus(SARS—CoV)induces apoptosis．we studied the SARS—CoV infected Vero E6 cells by agarose gel electrophoresis．indirect fluorescent staining for infection an d Hoechst 33528 staining for nuclei，as well as by flow cytometry．It was found that SARS—C0V infected Vero E6 cells showed typical apoptosis characteristics．Au the typical apoptotic ceHs were in late—infection period．Most of the cells with cytopathic effect(CPE)showed nuclei witll condensed chromatin or fragmented into apoptotic bodies．It was suggested that SARS—CoV infection induces apoptosis of Vero E6．
SARS-CoV Infection Induces Apoptosis of Vero E6
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Abstract
Abstract：To determine if SARS—associated coronavirus(SARS—CoV)induces apoptosis．we studied the SARS—CoV infected Vero E6 cells by agarose gel electrophoresis．indirect fluorescent staining for infection an d Hoechst 33528 staining for nuclei，as well as by flow cytometry．It was found that SARS—C0V infected Vero E6 cells showed typical apoptosis characteristics．Au the typical apoptotic ceHs were in late—infection period．Most of the cells with cytopathic effect(CPE)showed nuclei witll condensed chromatin or fragmented into apoptotic bodies．It was suggested that SARS—CoV infection induces apoptosis of Vero E6．
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Proportional views
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