Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province

DING Ming; FANG Qi; ZHANG Li-zhen; YANG Lu-ming; Wang Ji-ling; LI Yan-fang; ZHANG Zhong-kai

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February 2004

DING Ming, FANG Qi, ZHANG Li-zhen, YANG Lu-ming, Wang Ji-ling, LI Yan-fang and ZHANG Zhong-kai. Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province[J]. Virologica Sinica, 2004, 19(1): 76-78.

Citation: DING Ming, FANG Qi, ZHANG Li-zhen, YANG Lu-ming, Wang Ji-ling, LI Yan-fang, ZHANG Zhong-kai. Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province .VIROLOGICA SINICA, 2004, 19(1) : 76-78.

云南五个不同地区烟草花叶病毒外壳蛋白基因的序列比较*

张丽珍 ¹ ,
杨录明 ¹ ,
汪继玲 ¹ ,
李滟芳 ² ,
张仲凯 ² ,

1.
1. 云南省农业科学院生物技术研究所，云南昆明 650223
2.
云南红河卷烟厂，云南弥勒 652300

摘要
- 烟草花叶病毒
- , 外壳蛋白基因
- , 克隆
- , 序列分析

Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province

1.
1. Institute of Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650223,China
2.
Honghe Cigarette Factory, Mile 652300, China

Corresponding author: ZHANG Zhong-kai,

Abstract

Five Tobacco mosaic virus isolates, obtained from tobacco leaves showing typical symptom in Qujing, Honghe, Dali, Chuxiong and Yuxi in Yunnan province, were selected and studied from 637 TMV samples. Using a pair of primers specific for TMV-U1 strain, a specific fragment of 530bp including the TMV coat protein gene was amplified using IC-PCR. The products of PCR were cloned and sequenced. The nucleotide and amino acid sequences of the coat protein of the five isolates were found to be very similar each other and have more than 90% sequence identity with TMV-U1, TMV-B, TMV-P, TMV-FUJIAN, although minor differences existed among them. The results showed that the five isolates from Yunnan province belong toTMV-U1 strain.
- Tobacco mosaic virus
- , CP gene
- , Cloning
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province

Corresponding author: ZHANG Zhong-kai,

1. 1. Institute of Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650223,China
2. Honghe Cigarette Factory, Mile 652300, China

Abstract: Five Tobacco mosaic virus isolates, obtained from tobacco leaves showing typical symptom in Qujing, Honghe, Dali, Chuxiong and Yuxi in Yunnan province, were selected and studied from 637 TMV samples. Using a pair of primers specific for TMV-U1 strain, a specific fragment of 530bp including the TMV coat protein gene was amplified using IC-PCR. The products of PCR were cloned and sequenced. The nucleotide and amino acid sequences of the coat protein of the five isolates were found to be very similar each other and have more than 90% sequence identity with TMV-U1, TMV-B, TMV-P, TMV-FUJIAN, although minor differences existed among them. The results showed that the five isolates from Yunnan province belong toTMV-U1 strain.