SARS患者病理尸检肺组织样品分离病毒出现细胞病变的Hep2 培养细胞,按常规制作超薄切片,透射电镜下观察。电镜下,检出在感染细胞内复制、组装的呼肠孤病毒及其包涵体。病毒粒子衣壳立体对称、无包膜、直径在60~80nm。成熟病毒粒子核心致密常排列呈晶格状,不成熟病毒粒子核心空亮。数目不等的上述两种病毒粒子、长短不等的微管样结构和病毒浆常在核旁胞质内组成大小不等、无定形的病毒包涵体。此发现进一步提供了呼肠孤病毒感染有可能与SARS相关的形态学依据。

To identify the pathogen isolated from lung tissue of a patient died from SARS by using electron microscope. Sample of lung tissue taken from autopsy of the SARS patient was inoculated onto Hep2 cultured cells. Any cultured cells exhibiting cytopathic effect were processed by routine techniques for ultrathin sections. Electron microscopic examination revealed the characteristic morphological features of reovirus. The virus particles showed in different mature stages, the matured particles with dense core and the incompletely assembled virus particles with lucent core. Both of them have capsid about 14nm in thickness with a size of virions about 60-80nm in diameter. Most of the virions were found in large clusters arranged in crystalline array and associated with the viral plasm and microtubular-like structure to form cytoplasmic inclusion bodies of different size and shape. In this study, we have provided further morphological evidence that reovirus may be associated with SARS. However, the correlation of the