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Volume 20 Issue 5
October 2005

    JANG Yi-jun, JIANG Ping*, YANG Xiao-wei, TANG Jing-yuan, LI Yu-feng and LI Yong-dong. Construction and Indentification of Recombinant Adenovirus Containing Multiple Antigen Epitopes of Swine Foot-and-Mouth Disease Virus[J]. Virologica Sinica, 2005, 20(5): 511-514.
    Citation: JANG Yi-jun, JIANG Ping*, YANG Xiao-wei, TANG Jing-yuan, LI Yu-feng, LI Yong-dong. Construction and Indentification of Recombinant Adenovirus Containing Multiple Antigen Epitopes of Swine Foot-and-Mouth Disease Virus .VIROLOGICA SINICA, 2005, 20(5) : 511-514.
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    猪口蹄疫病毒多抗原表位重组腺病毒的构建与鉴定

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      南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏南京,210095

    • 本研究设计构建了含有猪O 型口蹄疫病毒VP1 (21260)2(1412160)2(2002213) 位氨基酸的基因的重组腺病毒质粒pAd2VP ,经PacI 酶切后转染HEK2293A 细胞,3 次噬斑纯化获得了重组腺病毒rAd2VP。该重组腺病毒于HEK2293A 细胞连续传代至20 代效价稳定,TCID50为10210 / mL 。RT2PCR 检测证明目的基因在mRNA 水平上可有效表达;应用O 型口蹄疫病毒标准阳性血清进行间接荧光抗体试验,在rAd2VP 感染的HEK2293A 细胞的胞质可见清晰荧光。证明该重组腺病毒对VP1 (21260)2(1412160)2(2002213) 位氨基酸的基因进行了成功的表达,从而为FMDV 多抗原表位腺病毒活载体疫苗的研究奠定了基础

    Construction and Indentification of Recombinant Adenovirus Containing Multiple Antigen Epitopes of Swine Foot-and-Mouth Disease Virus

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    • A recombinant replication defective Human adenovius serotype5 plasmid pAd2VP ,containing amino acids (21260)2(1412160)2(2002213) coding region of swine foot and mouth disease virus serotype O-VP1 , was const ructed using the method of homologous recombination in E.coli BJ5183. After the recombinant plasmid pAd2VP linearized with PacI t ransferred into HEK293A cell , the recombinant virus was isolated and purified in HEK2293A cells by t hree times plaque purification . This recombinant adenovirus could be stably passaged in HEK2293A cells and TCID50 was 10
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        沈阳化工大学材料科学与工程学院 沈阳 110142

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      Construction and Indentification of Recombinant Adenovirus Containing Multiple Antigen Epitopes of Swine Foot-and-Mouth Disease Virus

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      Abstract: A recombinant replication defective Human adenovius serotype5 plasmid pAd2VP ,containing amino acids (21260)2(1412160)2(2002213) coding region of swine foot and mouth disease virus serotype O-VP1 , was const ructed using the method of homologous recombination in E.coli BJ5183. After the recombinant plasmid pAd2VP linearized with PacI t ransferred into HEK293A cell , the recombinant virus was isolated and purified in HEK2293A cells by t hree times plaque purification . This recombinant adenovirus could be stably passaged in HEK2293A cells and TCID50 was 10

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