Molecular characterization of the genome segment S9 of Rice gall dwarf virus

FAN Guo-cheng; WU Zu-jian; HUANG Ming-hua; LIAN Jun; DONG Liang; LIN Qi-ying; XIE Lian-hui

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October 2005

FAN Guo-cheng, WU Zu-jian, HUANG Ming-hua, LIAN Jun, DONG Liang, LIN Qi-ying and XIE Lian-hui. Molecular characterization of the genome segment S9 of Rice gall dwarf virus[J]. Virologica Sinica, 2005, 20(5): 539-542.

Citation: FAN Guo-cheng, WU Zu-jian, HUANG Ming-hua, LIAN Jun, DONG Liang, LIN Qi-ying, XIE Lian-hui. Molecular characterization of the genome segment S9 of Rice gall dwarf virus .VIROLOGICA SINICA, 2005, 20(5) : 539-542.

水稻瘤矮病毒基因组S9片段的基因结构特征

范国成 ^1,2 ,
吴祖建 ^1* ,
黄明华 ³ ,
练君 ³ ,
梁栋 ³ ,
林奇英 ¹ ,
谢联辉 ¹

1.
1.福建福州福建农林大学植物病毒研究所 2.福建省农业科学院果树研究所 3.广东省信宜市农作物病虫测报站广东信宜

摘要

应用RT-PCR技术克隆了水稻瘤矮病毒(RGDV)中国广东信宜分离物(RGDV-C)的基因组S9片段,测定了全序列并进行了生物信息学分析。结果表明,RGDV-C S9片段全长共有1202bp(登录号AY556483),含有一个长的开放阅读框,这一开放阅读框编码一个由323个氨基酸残基组成的多肽,推测分子量约35.6kDa,与泰国分离物(RGDV-T)的全序列相比,它们的核苷酸长度相等,核苷酸同源性为98.1%,氨基酸同源性为98.5%。RGDV S9片段编码的Pns9蛋白在植物呼肠孤病毒属内未发现同源蛋白,其功能尚待确定。利用NCBI的BLAST查找与比较,发现Pns9与伯氏疏螺旋体(Borrelia burgdorferi)ATP依赖的Clp蛋白水解酶组分[ATP-dependent Clp prote-ase proteolytic component(clpP-1)]有21.8%的氨基酸序列同源性。
- 水稻瘤矮病毒
- , 基因组S9片段
- , 序列

Molecular characterization of the genome segment S9 of Rice gall dwarf virus

Abstract

The full-length cDNA of the genome segment(S9) of Rice gall dwarf virus Xinyi isolate(RGDV-C) was cloned and the complete nucleotide sequence was determined.The results showed that S9 had 1202 nucleotides(AY556483) encoding a polypeptide of 323 amino acids with an Mr of 35.6kDa.The S9 had the same genomic organization as that of RGDV Thailand isolate(RGDV-T) and shared 98.1% nucleotide and 98.5% amino acid sequence identity.No equivalent protein of Pns9 encoded by RGDV S9 ORF was found in other members of the genus Phythoreovirus.Pns9 shared 21.8% amino acid sequence identity with ATP-dependent Clp protease proteolytic component from Borrelia burgdorferi.
- Rice gall dwarf virus
- , Genome segment 9
- , Sequence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Molecular characterization of the genome segment S9 of Rice gall dwarf virus

Abstract: The full-length cDNA of the genome segment(S9) of Rice gall dwarf virus Xinyi isolate(RGDV-C) was cloned and the complete nucleotide sequence was determined.The results showed that S9 had 1202 nucleotides(AY556483) encoding a polypeptide of 323 amino acids with an Mr of 35.6kDa.The S9 had the same genomic organization as that of RGDV Thailand isolate(RGDV-T) and shared 98.1% nucleotide and 98.5% amino acid sequence identity.No equivalent protein of Pns9 encoded by RGDV S9 ORF was found in other members of the genus Phythoreovirus.Pns9 shared 21.8% amino acid sequence identity with ATP-dependent Clp protease proteolytic component from Borrelia burgdorferi.