DUAN Yan-Ping, CHEN Xiu-Zhu and Wu Yuan-Ming. Detection of the Antigen of Human Cytomegalovirus Isolates in Cell Culture by Indirect Immunofluorescent Assay Using Monoclonai Antibody[J]. Virologica Sinica, 1990, 5(1).
Citation:
DUAN Yan-Ping, CHEN Xiu-Zhu, Wu Yuan-Ming.
Detection of the Antigen of Human Cytomegalovirus Isolates in Cell Culture by Indirect Immunofluorescent Assay Using Monoclonai Antibody .VIROLOGICA SINICA, 1990, 5(1)
: 36.
单克隆抗体间接免疫荧光法检测巨细胞病毒分离培养中的抗原
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1.
同济医科大学微生物学教研室
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2.
中国科学院武汉病毒研究所 武汉
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摘要
应用抗巨细胞病毒(HCMV)蛋白抗原(分子量为20千道尔顿)的单克隆抗体(McAb-20k)和HCMV IgG特异性阳性血清以间接免疫荧光试验检测28例器官移植病人尿标本接种人胚肺细胞后的HCMV感染情况,结果前法于接种后48小时检测到HCMV阳性病人9例,后者于接种6天检测到阳性病人11例。与病毒分离结果相比较,两法的敏感性分别为81.8％和90.9％,特异性相应为100％和94.12％,符合率均为92.9％,比病毒分离提前数天至数周作出诊断,重复性良好。因此,抗HCMV蛋白抗原的单克隆抗体间接免疫荧光法是一种有效的、早期快速而又敏感特异的诊断方法,操作简便,既使没有单抗,可用HCMV IgG阳性血清代替,也可取得较好效果,值得在一般实验室推广应用。
Detection of the Antigen of Human Cytomegalovirus Isolates in Cell Culture by Indirect Immunofluorescent Assay Using Monoclonai Antibody
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Abstract
Two indirect immunofluorescent assays(IIFA), using a monoclonal antibody directed against protein antigen (McAb-20k)and specific positive sera of human cytomegalovirus(HCMV)respectively, were applied for the rapid detection of the virus from fifty urine specimens(28 cases)inoculated onto human embryonic fibroblast. By the former procedure g of 28 cases were positive at 48 hours postinfection and all of them developed cytopathic effect; by the latter 11 were positive at 6 days postinfection and only 10 cases...
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Proportional views
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