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2015年30卷2期

Viruses are leading causes of different types of human cancers, accounting for about 20% of total cases. Seven viruses are currently considered oncogenic viruses, including hepatitis B virus (HBV), hepatitis C virus (HCV), human papillomavirus (HPV), Epstein Barr virus (EBV), human herpes virus 8 (HHP8), Merkel cell polyomavirus (MCPyV), and human T-lymphotropic virus type 1 (HTLV-1). The molecular mechanisms of viral oncogenesis are complex and may involve the induction of chronic inflammation, disruption of host genetic and epigenetic integrity and homeostasis, interference with cellular DNA repair mechanisms resulting in genome instability and cell cycle dysregulation. Genetic and epigenetic alterations induced by infection and replication of oncogenic viruses may lead to the appearance and proliferation of cancer stem cells, which are important for the initiation, progression, metastasis, relapse, and chemotherapy resistance of cancers. The cover illustrates the seven oncoviruses that could lead to human cancer.

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Editorial

Oncogenic viruses and cancer

Guangxiang George, hsiung James

2015, 30(2): 83 doi: 10.1007/s12250-015-3599-y

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This special issue of the journal is dedicated to the important topic of oncogenic viruses and cancer. It contains seven review articles covering all known oncogenic viruses except for human T-lymphotropic virus type 1 (HTLV-1). These review articles are contributed by experts on specific viruses and their associated human cancers. Viruses account for about 20% of total human cancer cases. Although many viruses can cause various tumors in animals, only seven of them are associated with human cancers and are currently considered oncogenic viruses. These viruses include hepatitis B virus (HBV), hepatitis C virus (HCV), human papillomavirus (HPV), Epstein Barr virus (EBV), human herpes virus 8 (HHV8), Merkel cell polyomavirus (MCPyV), and HTLV-1. HBV and HCV cause approximately 80% of hepatocellular carcinoma (HCC), the most common cancer of the liver. High-risk HPV strains are the major causes of cervical cancer and other ano-genital neoplasms as well as a significant proportion of head and neck tumors. EBV is associated with nasopharyngeal carcinoma, Hodgkin’s lymphoma, and Burkitt’s lymphoma. HHV8 (also called Kaposi’s sarcoma-associated herpesvirus,KSHV) is responsible for Kaposi’s sarcoma often found in patients with acquired immunodeficiency syndrome (AIDS). MCPyV causes Merkel cell carcinoma and HTLV-1 is the causative agent of adult T-cell lymphoma.
Review

Genetic and epigenetic alterations in hepatitis B virus-associated hepatocellular carcinoma

Yongjun Tian, Jing-hsiung James Ou*

2015, 30(2): 85 doi: 10.1007/s12250-015-3582-7

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Hepatitis B virus (HBV) is a major cause of hepatocellular carcinoma (HCC). Its chronic infection can lead to chronic liver inflammation and the accumulation of genetic alterations to result in the oncogenic transformation of hepatocytes. HBV can also sensitize hepatocytes to oncogenic transformation by causing genetic and epigenetic changes of the host chromosomes. HBV DNA can insert into host chromosomes and recent large-scale whole-genome sequencing studies revealed recurrent HBV DNA integrations sites that may play important roles in the initiation of hepatocellular carcinogenesis. HBV can also cause epigenetic changes by altering the methylation status of cellular DNA, the post-translational modification of histones, and the expression of microRNAs. These changes can also lead to the eventual hepatocellular transformation. These recent findings on the genetic and epigenetic alterations of the host chromosomes induced by HBV opened a new avenue for the development of novel diagnosis and treatments for HBV-induced HCC.

Model systems to study the life cycle of human papillomaviruses and HPV-associated cancers

Louise T. Chow*

2015, 30(2): 92 doi: 10.1007/s12250-015-3600-9

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The prevalent human papillomaviruses (HPVs) infect either cutaneous or mucosal epithelium. Active Infections lead to epithelial hyperprolifeation and are usually cleared in healthy individuals within a year. Persistent infections in the anogenital tracts by certain high-risk genotypes such as HPV-16, HPV-18 and closely related types, can progress to high grade dysplasias and carcinomas in women and men, including cervical, vulva, penile and anal cancers. A signifiant fraction of the head and neck cancers are also caused by HPV-16. The viral oncogenes responsible for neoplastic conversion are E6 and E7 that disrupt the pathways controlled by the two major tumor suppressor genes, p53 and members of pRB family. Because HPV cannot be propagated in conventional submerged monolayer cell cultures, organotypic epithelial raft cultures that generate a stratifid and differentiated epithelium have been used to study the viral life cycle. This article describes several systems to examine aspects of the viral productive phase, along with the advantages and limitations. Animal model systems of HPV carcinogenesis are also briefl described.

Recent advances in the study of HPV-associated carcinogenesis

2015, 30(2): 101 doi: 10.1007/s12250-015-3586-3

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Human papillomaviruses (HPVs) cause virtually all cervical cancers, the second leading cause of death by cancer among women, as well as other anogenital cancers and a subset of head and neck cancers. Approximately half of women, who develop cervical cancer die from it. Despite the optimism that has accompanied the introduction of prophylactic vaccines to prevent some HPV infections, the relatively modest uptake of the vaccine, especially in the developing world, and the very high fraction of men and women who are already infected, means that HPV-associated disease will remain as a significant public health problem for decades. In this review, we summarize some recent findings on HPV-associated carcinogenesis, such as miRNAs in HPV-associated cancers, implication of stem cells in the biology and therapy of HPV-positive cancers, HPV vaccines, targeted therapy of cervical cancer, and drug treatment for HPV-induced intraepithelial neoplasias.

The role of Epstein-Barr virus infection in the pathogenesis of nasopharyngeal carcinoma

2015, 30(2): 107 doi: 10.1007/s12250-015-3592-5

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Nasopharyngeal carcinoma (NPC) is closely associated with Epstein-Barr virus (EBV) infection. EBV episomes are detected in almost all NPC cells. The role of EBV in NPC pathogenesis has long been postulated but remains enigmatic. In contrast to infection of B lymphocytes, EBV infection does not directly transform nasopharyngeal epithelial cells into proliferative clones with malignant potential. EBV infection of normal pharyngeal epithelial cells is predominantly lytic in nature. Genetic alterations in premalignant nasopharyngeal epithelium, in combination with inflammatory stimulation in the nasopharyngeal mucosa, presumably play essential roles in the establishment of a latent EBV infection in infected nasopharyngeal epithelial cells during the early development of NPC. Establishment of latent EBV infection in premalignant nasopharyngeal epithelial cells and expression of latent viral genes, including the BART transcripts and BART-encoded microRNAs, are crucial features of NPC. Expression of EBV genes may drive further malignant transformation of premalignant nasopharyngeal epithelial cells into cancer cells. The difficulties involved in the establishment of NPC cell lines and the progressive loss of EBV epsiomes in NPC cells propagated in culture strongly implicate the contribution of host stromal components to the growth of NPC cells in vivo and maintenance of EBV in infected NPC cells. Defining the growth advantages of EBV-infected NPC cells in vivo will lead to a better understanding of the contribution of EBV infection in NPC pathogenesis, and may lead to the identification of novel therapeutic targets for NPC treatment.

Polyomavirus interaction with the DNA damage response

2015, 30(2): 122 doi: 10.1007/s12250-015-3583-6

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Viruses are obligate intracellular parasites that subvert cellular metabolism and pathways to mediate their own replication—normally at the expense of the host cell. Polyomaviruses are a group of small DNA viruses, which have long been studied as a model for eukaryotic DNA replication. Polyomaviruses manipulate host replication proteins, as well as proteins involved in DNA maintenance and repair, to serve as essential cofactors for productive infection. Moreover, evidence suggests that polyomavirus infection poses a unique genotoxic threat to the host cell. In response to any source of DNA damage, cells must initiate an effective DNA damage response (DDR) to maintain genomic integrity, wherein two protein kinases, ataxia telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR), are major regulators of DNA damage recognition and repair. Recent investigation suggests that these essential DDR proteins are required for productive polyomavirus infection. This review will focus on polyomaviruses and their interaction with ATMand ATR-mediated DNA damage responses and the effect of this interaction on host genomic stability.

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

2015, 30(2): 130 doi: 10.1007/s12250-015-3595-2

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It has now been over twenty years since a novel herpesviral genome was identified in Kaposi’s sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi’s sarcoma-associated herpesvirus (KSHV; also known as human herpesvirus 8 (HHV- 8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.
Research Article

EV71 感染与儿童口咽粘膜液中病毒IgG相关

薛京昌, 李么明, 徐晓怡, 俞杰, 闫虎, 鄢慧民

2015, 30(2): 146 doi: 10.1007/s12250-014-3555-2

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SARS冠状病毒共编码八个附属蛋白,它们的功能目前还未完全研究透彻。其中,SARS冠状病毒蛋白6(P6)已被报道能够增强病毒的复制,抑制宿主干扰素信号通路。通过酵母双杂技术,我们从人的脾脏cDNA文库中筛选出了八个能够与P6相互作用的蛋白。进一步,我们选择了干扰素信号通路的一个调控蛋白N-Myc interactor (Nmi),并在细胞水平证明了Nmi能够与P6相互作用。同时,我们还发现,P6能够介导Nmi通过泛素化蛋白酶体途径降解。这一发现为解释SARS冠状病毒P6抑制干扰素信号通路,促进病毒自身在宿主细胞中存活提供了一个新的机制。

SARS冠状病毒蛋白6介导Nmi蛋白的泛素化蛋白酶体降解

程唯佳, 陈世优, 李瑞玲, 陈宇, 汪敏, 郭德银

2015, 30(2): 153 doi: 10.1007/s12250-015-3581-8

[HTML全文] [PDF 1237 KB] Springerlink
SARS冠状病毒共编码八个附属蛋白,它们的功能目前还未完全研究透彻。其中,SARS冠状病毒蛋白6(P6)已被报道能够增强病毒的复制,抑制宿主干扰素信号通路。通过酵母双杂技术,我们从人的脾脏cDNA文库中筛选出了八个能够与P6相互作用的蛋白。进一步,我们选择了干扰素信号通路的一个调控蛋白N-Myc interactor (Nmi),并在细胞水平证明了Nmi能够与P6相互作用。同时,我们还发现,P6能够介导Nmi通过泛素化蛋白酶体途径降解。这一发现为解释SARS冠状病毒P6抑制干扰素信号通路,促进病毒自身在宿主细胞中存活提供了一个新的机制。